Plasmid

Part:BBa_K3904318:Design

Designed by: Bernadeta Aleksandravičiūtė   Group: iGEM21_Vilnius-Lithuania   (2021-09-30)


pET-28a(+) with N-His tag without any additional amino acids between tag and protein sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5140
    Illegal XbaI site found at 5030
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5140
    Illegal NheI site found at 5101
    Illegal NotI site found at 5165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5140
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5134
    Illegal XhoI site found at 5174
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5140
    Illegal XbaI site found at 5030
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5140
    Illegal XbaI site found at 5030
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


Design Notes

.


Source

Synthetic.

References